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Primer sequences for qRT-PCR
| Primer target | Sequence |
|---|---|
| Gapdh | Forward: 5ʹ-TTC ACC ACC ATG GAG AAG GC-3ʹ |
| Reverse: 3ʹ-GGC ATG GAC TGT GGT CAT GA-5ʹ | |
| Kdm6a | Forward: 5ʹ-TTC CTC GGA AGG TGC TAT TCA-3ʹ |
| Reverse: 3ʹ-GAG GCT GGT TGC AGG ATT CA-5ʹ | |
| Xist | Forward: 5ʹ-CAGAGTAGCGAGGACTTGAAGAG-3ʹ |
| Reverse: 3ʹ-GCTGGTTCGTCTATCTTGTGGG-5ʹ |
