Fig. 2.

Screening and verification of candidate LILRB3 upstream target miRNAs, and miR-103a-2-5p was selected. A Venn diagram of the target miRNAs of LILRB3 based on miRDB, TargetScan, miRWalk. B A potential miR-504a-3p, miR-5702, miR-8077, and miR-103a-2-5p binding site in the 3ʹ-UTR of LILRB3 was predicted using TargetScan (https://www.targetscan.org/). The expression of LILRB3 at the protein (C) and mRNA (D) levels was measured by qRT-PCR and western blotting analysis. (E, F) The sequence of luciferase reporter vector. Luciferase reporter gene assays were performed to confirm the direct targeting of LILRB3 to miR-103a-2-5p. G The qRT-PCR assay examined the expression of miR-103a-2-5p in HEK293T cells transfected with control miRNA (miR-NC) or miR-103a-2-5p. H Cells were transfected with either luciferase reporter vector containing the LILRB3-3ʹUTR wild-type or mutant in the target sites in the presence or absence of precursors of miR for 48 h followed by measuring the luciferase activity. All results are presented as the mean ± SD, *P < 0.05, **P < 0.01. Cell experiments were performed three times independently