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. 2024 Mar 14;17:6. doi: 10.1186/s13072-024-00529-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — TRIM29 interacts with TP63 and regulates the expression of the TP63 cluster. A Eigengene of TP63 cluster significantly correlates with the expression of TRIM29. B TP63 expression significantly correlates with the expression of TRIM29 in four PRAD datasets. C Comparison of TP63 and TRIM29 knockdowns (KDs). GSEA plots evaluating the TP63 cluster signature upon TRIM29 knockdown in RWPE-1 (prostate basal epithelium, left-top) and HMEC (GSE71375, basal breast epithelium, left-bottom). Venn diagram showing the overlap of differentially expressed TP63 cluster genes upon TP63 and TRIM29 knockdown in the RWPE-1 cells (right). NES Normalized enrichment scores. D Comparison of TP63 and TP63 + TRIM29 overexpression (OEs). GSEA plots evaluating the TP63 cluster signature upon TRIM29 overexpression in PC3 (PRAD, left-top) and TP63 and TRIM29 simultaneous overexpression in PC3 (PRAD, left-bottom). Venn diagram showing the overlap of differentially expressed TP63 cluster genes upon TP63 overexpression and TP63 and TRIM29 simultaneous overexpression in PC3 cells (right). E ChIP-seq profiles of H3K27ac (top track) and TP63 (bottom track) in the RWPE-1 cell line of the TRIM29 gene. Enhancer 1 of TRIM29 in a red polygon. F Immunoblot (IB) analysis of knockdown (KD) of TP63 and TRIM29 (left) and TP63 and TRIM29 overexpression (OE, right) in the RWPE-1 cells. G Immunoblot (IB) analysis of overexpression (OE) of TP63 and TRIM29 in PRAD cell lines: LNCaP, 22Rv1, and PC3. H Interaction between TRIM29 and TP63 was detected by co-immunoprecipitation (co-IP) in the PC3 cells overexpressing TRIM29-FLAG and TP63. I Overexpression (OE) of TRIM29 does not lead to the relocation of TP63 from the nucleus to the cytoplasm. Immunocytochemistry staining of TP63 in the RWPE-1 cells overexpressing TRIM29. J Immunoblot (IB) analysis of overexpression (OE) of TP63 and TRIM29 simultaneously in PC3 cells