TABLE 2.
Genetic changes distinguishing the 3′-half genomes of BK28 and H824a
| Segment | Length (nt/aa) | No. (%) of:
|
Comments regarding changes in H824 relative to BK28 | |
|---|---|---|---|---|
| nt changes | aa changes | |||
| Δpol | 314/104 | 2 (0.6) | 1 (1.0) | |
| vif | 642/214 | 5 (0.8) | 3 (1.4) | One new N-linked glycosylation site |
| vpx | 336/112 | 3 (0.9) | 0 (0.0) | |
| vpr | 291/97 | 1 (0.3) | 1 (0.5) | One N-linked glycosylation site lost |
| tat1 | 296/99 | 3 (1.0) | 3 (3.0) | Mutation in consensus splice donor led to amino acid change |
| rev1 | 70/23 | 2 (2.9) | 1 (4.2) | Mutation in consensus splice donor led to amino acid change |
| gp120 | 1,539/513 | 25b (1.6) | 20 (3.9) | Five new N-linked glycosylation sites; neutralization site in V2 altered |
| tat2 | 97/32 | 1 (1.0) | 1 (3.1) | Mutation in consensus splice donor led to reversion of stop codon and elongation of TM |
| rev2 | 254/85 | 2 (0.8) | 1 (1.2) | Mutation in consensus splice donor led to reversion of stop codon and elongation of TM |
| gp32/41 | Stop codon reverted to encode gp41 | |||
| nef | 741/247 | 9c (1.2) | 25b (10.1) | Repair of a frameshift mutation, resulting in replacement of 15 amino acids and extension of the protein by 16 amino acids |
| ΔLTR | 747 | 9 (1.2) | One base change in Sp1 site brings it closer to consensus; another Sp1 site has a 1-base deletion; TATAA consensus changed to CATAA | |
a
Δ, analysis of only a portion of the gene or LTR segment; nt, nucleotide; aa, amino acid.
b
In addition to the point mutations tallied, one four-codon deletion and a one-codon insertion were also detected.
c
Relative to the SIV database consensus, a deletion was present in BK28 but was repaired in H824.