FIG. 3.

Coxsackie B virus attachment to mCAR on transfected CHO cells. Confluent monolayers of CHO-mCAR or control CHO-al2 cells were incubated with radiolabeled CB3 or CB4 (29,000 cpm) for 4 h at room temperature and then washed and dissolved for scintillation counting. Results with clone m2 are shown; similar results were obtained with clone m1. Some monolayers were preincubated with recombinant adenovirus 5 knob domains (produced in E. coli [0.7 μg]) before exposure to radiolabeled virus. Results for triplicate samples (mean virus bound [counts per minute] + 1 standard deviation [error bar]) are shown.