FIG. 4.

Coxsackievirus production by transfected CHO cells. CHO-mCAR and CHO-al2 monolayers were exposed to CB3 or CB4 (10 PFU/cell) for 1 h at room temperature and then monolayers were washed and incubated at 37°C for 1 h (0 days), 1 day, or 2 days. Monolayers were frozen and thawed to release virus, and then plaque assays were performed. The figure shows the mean virus titers for triplicate cultures.