FIG. 5.

Adenovirus interaction with mCAR on transfected CHO cells. (A) Virus attachment. Cell monolayers were incubated with radiolabeled adenovirus 2 (20,000 cpm) for 1 h at room temperature and then washed and dissolved for scintillation counting. Some monolayers were preincubated with adenovirus 2 fiber (5 μg) for 1 h and then washed before exposure to radiolabeled virus. (B) Knob attachment. Monolayers were incubated with ¹²⁵I-labeled adenovirus 5 knob domains (produced in insect cells) for 1 h at room temperature and then washed and dissolved for scintillation counting. Results for triplicate samples (mean virus or knob protein bound [in counts per minute] + 1 standard deviation [error bar]) are shown.