FIG. 6.

Adenovirus-mediated gene transfer. Duplicate monolayers of CHO-al2, CHO-mCAR, or CHO cells transfected with human CAR cDNA (CHO-hCAR) were exposed to Ad.CMV-βgal for 1 h at room temperature, and then monolayers were washed. After incubation at 37°C for 40 h, β-galactosidase activity was detected by in situ staining with X-Gal. Some monolayers were incubated with 1.5 μg of purified adenovirus 2 fibers (+) before exposure to virus. Results with clone m2 are shown; similar results were obtained with clone m1.