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CAR mRNA expression in human and murine tissues. Multiple-tissue Northern blots (Clontech) containing 2 μg of poly(A)+ RNA from each of the indicated tissues were probed with human CAR and mCAR cDNA as described in Materials and Methods. Positions of marker RNAs are indicated in kilobases. Hybridization with a human actin probe confirmed the presence of equivalent amounts of RNA in each lane.
