Table 1.
Target genes and primer sequences used for PCR.
| Bacteria | Target gene | Primer name | Sequence (5’–3’) | Size (bp) | References |
|---|---|---|---|---|---|
| B.burgdorferi | 5S-23S rRNA IGS | F1 R1 F2 R2 |
CGACCTTCTTCGCCTTAAAGC TAAGCTGACTAATACTAATTACCC TCCTAGGCATTCACCATA GAGTTCGCGGGAGA |
255 | (21) |
| B.miyamotoi | glpQ | F1 R1 F2 R2 |
CACCATTGATCATAGCTCACAG CTGTTGGTGCTTCATTCCAGTC GCTAGTGGGTATCTTCCAGAAC CTTGTTGTTTATGCCAGAAGGGT |
424 | (22) |
| SFGR | ompA | Rr190.70 Rr190.701 Rr190.70 Rr190.602 |
ATGGCGAATATTTCTCCAAAA GTTCCGTTAATGGCAGCATCT ATGGCGAATATTTCTCCAAAA AGTGCAGCATTGGCTCCCCCT |
533 | (23) |
| SFGR | gltA | Cs2d CsendR Rpcs877 Rpcs1258 |
ATGACCAATGAAAATAATAAT CTTATACTCTCTATGTACA GGGGACCTGCTCACGGCGG ATTGCAAAAAGTACAGTGAACA |
341 | (8) |
| SFGR | gltA | F R P |
GTGAATGAAAGATTACACTATTTAT GTATCTTAGCAATCATTCTAATAGC 6-FAM-CTATTATGCTTGCGGCTGTCGGTTC-TAMRA |
166 | (24) |
| AP | 16S rRNA | AP-F AP-R |
GTCGAACGGATTATTCTTTATAGCTTG TATAGGTACCGTCATTATCTTCCCTAC |
389 | (25) |
| EC | 16S rRNA | ECC ECB H3 H1 |
AGAACGAACGCTGGCGGCAAGCC CGTATTACCGCGGCTGCTGGCA TATAGGTACCGTCATTATCTTCCCTAT CAATTGCTTATAACCTTTTGGTTATAAAT |
389 | (26, 27) |
| CB | IS1111 | F1 R1 F2 R2 |
TACTGGGTGTTGATATTGC CCGTTTCATCCGCGGTG GTAAAGTGATCTACACGA TTAACAGCGCTTGAACGT |
297 | (28) |
SFGR, spotted fever group rickettsia; AP, Anaplasma phagocytophilum; EC, Ehrlichia chaffeensis; CB, Coxiella burnetii.