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. 2024 Feb 29;18(10):7618–7632. doi: 10.1021/acsnano.4c00550

Figure 3.

Figure 3

Impact of AnCHNPs on DC maturation and migration, tested with BMDCs. DCs were incubated with AnCHNPs or CaCl2 (5 or 10 μg/mL). (a–c) Populations and mean fluorescence intensities (MFIs) of MHC-II+ and CD205+ cells (n = 3). (a) Quadrants showing the changes of MHC-II+ and CD205+ populations among DCs (CD11+). Histograms showing the MFI and population changes for (b) MHC-II+ and (c) CD205+ cells. (d) Transwell assay examining DC migration. B16F10-OVA cells with or without preirradiation (100 Gy) were seeded into the bottom chamber, while CFSE-labeled DCs were loaded into the inset. CFSE+ cells in the lower chamber at 24 h were quantified by flow cytometry (n = 3). (e) MFIs of CD80 on DCs when incubated with IL-10 (n = 3). Data are presented as the mean ± s.d. *, p < 0.05; **, p < 0.01; ***, p < 0.001.