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. 2024 Feb 29;18(10):7618–7632. doi: 10.1021/acsnano.4c00550

Figure 4.

Figure 4

Impact of AnCHNPs on DC maturation, tested in vitro with a coculture of BMDCs and B16F10-OVA (preirradiated, 100 Gy) in the presence of AnCHNPs or CaCl2 (5 or 10 μg/mL) (n = 3). (a) Quadrant graphs showing the changes of CD80+CD86+ and MHC-II+SIINFEKL-H-2Kb+ populations among DCs, analyzed by flow cytometry. In the control group, live B16F10-OVA cells were used in the coculture, and PBS was added into the incubation medium. (b) Bar graphs showing the frequencies of CD80+CD86+, CD40+, MHC-II+, and MHC-II+SIINFEKL-H-2Kb+ cells among DCs (n = 3). (c) Pro- (IL-6, IL-12, and TNF-α) and anti-inflammatory (IL-10) cytokines in the supernatant of the coculture, analyzed by ELISA (n = 3). Data are presented as the mean ± s.d. *, p < 0.05; **, p < 0.01; ***, p < 0.001.