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. 2024 Jan 12;3(3):421–430. doi: 10.1039/d3sd00246b

Detection results of RT-RPA for respective assay and readout type. Positive test outcome threshold determined by NTC signal intensity average plus three standard deviations.

Detection method 250 cps/rxn 100 cps/rxn 50 cps/rxn 25 cps/rxn 5 cps/rxn NTC
Singleplex RT-RPA with fluorescence 5/5 5/5 5/5 5/5 3/5 0/5
Duplex RT-RPA with fluorescence 5/5 5/5 5/5 5/5 2/5 0/5
Singleplex RT-RPA with LFA 5/5 5/5 5/5 5/5 0/5 0/5
Duplex RT-RPA with LFA 5/5 5/5 5/5 4/5 0/5 0/5