Detection results of RT-RPA for respective assay and readout type. Positive test outcome threshold determined by NTC signal intensity average plus three standard deviations.
| Detection method | 250 cps/rxn | 100 cps/rxn | 50 cps/rxn | 25 cps/rxn | 5 cps/rxn | NTC |
|---|---|---|---|---|---|---|
| Singleplex RT-RPA with fluorescence | 5/5 | 5/5 | 5/5 | 5/5 | 3/5 | 0/5 |
| Duplex RT-RPA with fluorescence | 5/5 | 5/5 | 5/5 | 5/5 | 2/5 | 0/5 |
| Singleplex RT-RPA with LFA | 5/5 | 5/5 | 5/5 | 5/5 | 0/5 | 0/5 |
| Duplex RT-RPA with LFA | 5/5 | 5/5 | 5/5 | 4/5 | 0/5 | 0/5 |