Table 1.
A. List of all O-linked glycans from colonic mucins from WT and Cyba mut mice. B. List of all O-linked glycans from ileal mucins from WT and Cyba mut mice.
| Name [1] | Tentative sequence | GU |
| 368-1PH | Fucα1-2Gal | 1.60 |
| 384–1 | Galβ1-3GalNAc | 1.79 |
| 384-2PH | GlcNacβ1-6Gal | 1.82 |
| 343-1PH | Galβ1-3Glc | 1.97 |
| 530–1 | Fucα1-2Galβ1-3GalNAc | 2.26 |
| 530-2PH | Galβ1-3(Fucα1–2)GlcNAc | 2.40 |
| 546-1PH | Galβ1-3GlcNAcβ1-6Gal | 2.62 |
| 587–1 | Galβ1–3[GlcNAcβ1–6]GalNAc | 2.68 |
| 667–1 | Galβ1–3[SO3-GlcNAcβ1–6]GalNAc | 2.90 |
| 675–1 | NeuAcα2-3Galβ1-3GalNAc | 2.99 |
| 733–1 | Fucα1-2Galβ1–3[GlcNAcβ1–6]GalNAc | 2.95 |
| 749–1 | Galβ1-3(Galβ1-3GlcNAcβ1–6)GalNAc | 3.43 |
| 749–2 | Galβ1-3(Galβ1-4GlcNAcβ1–6)GalNAc | 3.48 |
| 790–3 | GalNAcβ1-6Galβ1-3(GlcNAc1-6)GalNAc | 3.20 |
| 813–1 | Fucα1-2Galβ1-3(SO3-GlcNAcβ1–6)GalNAc | 3.33 |
| 878–1 | Galβ1-3(NeuAcα2–6)GlcNAcβ1-3GalNAc | 3.52 |
| 895–1 | Galβ1–3[Fucα1-2Galβ1-3GlcNAcβ1–6]GalNAc | 3.89 |
| 895–2 | Galβ1–3[Fucα1-2Galβ1-4GlcNAcβ1–6]GalNAc | |
| 895–3 | Fucα1-2Galβ1–3[Galβ1-3/4GlcNAcβ1–6]GalNAc | 3.89 |
| 975–1 | Galβ1–3[Fucα1-2Galβ1-3/4SO3-GlcNAcβ1–6]GalNAc | 3.96 |
| 1041–1 | Fucα1-2Galβ1–3[Fucα1-2Galβ1-3GlcNAcβ1–6]GalNAc | 4.08 |
| 1041–2 | Fucα1-2Galβ1–3[Fucα1-2Galβ1-4GlcNAcβ1–6]GalNAc | 4.28 |
| 1121–1 | Fucα1-2Galβ1–3[Fucα1-2Galβ1-3SO3-GlcNAcβ1–6]GalNAc | 4.32 |
| 1121–2 | Fucα1-2Galβ1–3[Fucα1-2Galβ1-4SO3-GlcNAcβ1–6]GalNAc | 4.42 |
| 1169–1 | NeuAc2-3GalNAcβ1-4(NeuAcα2–6)Galβ1-3GalNAc/GlcNAc | 5.00 |
| 1169–2 | NeuAc2-3/6GlcNAc/GalNAcβ1-3/4/6(NeuAcα2–6)Galβ1-3GalNAc/GlcNAc | 5.09 |
| 1128-1PH | NeuAc2-3Galβ1-3(NeuAcα2–6)GlcNAcβ1-6Gal | 5.22 |
| 1178–1 | GalNAcβ1-4Galβ1–3[Fucα1-2Galβ1-3/4SO3-GlcNAcβ1–6]GalNAc | 4.20 |
| 1186–1 | Fucα1-2Galβ1–3[NeuAcα2-6Galβ1-3GlcNAcβ1–6]GalNAc | 4.49 |
| 1243–1 | Galβ1–3[GalNAcβ1-4NeuAcα2-6Galβ1-3/4GlcNAcβ1–6]GalNAc | 4.89 |
| 1243–1 | Galβ1–3[GalNAβ1-4Galβ1-3(NeuAcα2-3/6)GlcNAcβ1–6]GalNAc | 5.28 |
| 1266–1 | Galβ1–3[NeuAcα2-3/6Galβ1-3/4(Fucα1–2)SO3-GlcNAcβ1–6]GalNAc | 5.28 |
| 1372–1 | GlcNAc/GalNAcβ1-4/6(NeuAcα2–3)Galβ1–3[NeuAcα2-3/6GlcNAcβ1–6]GalNAc | 5.28 |
| 1131–1 | NeuAcα2-6Galβ1–3[Galβ1-3(NeuAcα2–6)GlcNAcβ1–6]GalNAc | 5.95 |
| NeuAcα2-3/6Galβ1–3[Galβ1-4(NeuAcα2-3/6)GlcNAcβ1–6]GalNAc | 6.21 | |
| 1331-1P | NeuAcα2-6GalNAcβ1-4(NeuAcα2–6)Galβ1-3GlcNAcβ1-6Gal | 5.51 |
| 1372–2 | GalNAcβ1-4(NeuAcα2-3/6)Galβ1-3/4(NeuAcα2-3/6)GlcNAcβ1-3GalNAc | 5.83 |
| 1389–1 | Fucα1-2Galβ1–3[GlcNAcβ1-3(NeuAcα2–6)Galβ1-3GlcNAcβ1–6]GalNAc | 5.69 |
| 1469–1 | NeuAcα2-3/6(Fucα1–2)Galβ1–3[GalNAc/GlcNAcβ1-3/4/6Galβ1-3/4SO3-GlcNAcβ1–6]GalNAc | 5.38 |
| 1477–1 | Fucα1-2Galβ1–3[NeuAcα2-6Galβ1-3(NeuAcα2-3/6)GlcNAcβ1–6]GalNAc | 6.67 |
| 1535–1 | Galβ1–3[GalNAβ1-4(NeuAcα2–3)Galβ1-3(NeuAcα2-3/6)GlcNAcβ1–6]GalNAc | 6.94 |
| 1535–1 | Galβ1–3[NeuAcα2-6GlcNAcβ1-6(NeuAcα2–6)Galβ1-3/4GlcNAcβ1–6]GalNAc | 6.94 |
| 1535–1 | GalNAcβ1-4(NeuAcα2–6)Galβ1–3[Galβ1-3(NeuAcα2-3/6)GlcNAcβ1–6]GalNAc | 7.21 |
| 1681–1 | Fucα1-2Galβ1–3[GlcNAc/GalNAcβ1-3/4(NeuAcα2–6)Galβ1-3/4(NeuAcα2-3/6)GlcNAcβ1–6]GalNAc (4x dif isomers- dif in GalNAc/GlcNAc and galactose linkage) |
7.62/
7.91/ 8.14 |
| 1843–1 | NeuAcα2-3/6-, Fucα-GalNAcβ1-4Galβ1-3/4GlcNAc/GalNAcβ1-3/4/6Galβ1-3/4GlcNAcβ1-6Gal | 8.57 |
| 1843–2 | 8.86 | |
| 1892–1 | NeuAcα2-3/6-GlcNAcβ1-3/6Galβ1–3[GalNAcβ1-4Galβ1-3/4GlcNAcβ1-3/6Galβ1-3/4SO3-GlcNAcβ1–6]GalNAc | 6.10 |
| 2126–1 | NeuAcα2-3/6GalNAc/GlcNAcβ1-3/4/6(Fucα1–2)Galβ1–3[NeuAcα2-3/6)Galβ1-3GalNAc/GlcNAcβ1-3/4/6Galβ1-3SO3-GlcNAcβ1–6]GalNAc | 9.00 |
| Fucα1-2Galβ1-3(GalNAc/GlcNAcβ1-3/4[NeuAcα2-6Galβ1-3/4GalNAc/GlcNAcβ1-4/6(NeuAcα2-3/6)Galβ1-3/4SO3-GlcNAcβ1–6]GalNAc | 9.00 | |
| Fucα1-2Galβ1–3[GalNAc/GlcNAcβ1-4/6(NeuAcα2–3)Galβ1-3/4GalNAc/GlcNAcβ1-3/4/6Galβ1-3/4(NeuAcα2-3/6)SO3-GlcNAcβ1–6]GalNAc | 9.30 | |
| 2126–1 | NeuAcα2-3/6GalNAc/GlcNAcβ1-3/4/6(Fucα1–2)Galβ1–3[NeuAcα2-3/6Galβ1-3GalNAc/GlcNAcβ1-3/4/6Galβ1-4SO3-GlcNAcβ1–6]GalNAc | 9.99 |
| 2249–1 | (NeuAcα2-3/6)2-Galβ1-3/4(Fucα1–2)GlcNAcβ1-3/6[GalNAcβ1-4Galβ1-3/4GlcNAcβ1-3/6Galβ1-3/4GlcNAcβ1-3/6]GalNAc | 10.90 |
| Name [1] | Tentative sequence | GU |
| 368-1PH | Fucα1-2Gal | 1.59 |
| 384–1 | Galβ1-3GalNAc | 1.80 |
| 425–1 | GlcNAcβ1-6GalNAc | 1.82 |
| 505–1 | SO3-GlcNAcβ1-3GalNAc | |
| 343-1PH | Galβ1-3Glc | 1.96 |
| 530–1 | Fucα1-2Galβ1-3GalNAc | 2.26 |
| 472-P | NeuAcα2-3Gal | 2.40 |
| 505-P | Galβ1-3Galβ1-3Gal | 2.62 |
| 587–1 | Galβ1–3[GlcNAcβ1–6]GalNAc | 2.69 |
| 667–1 | Galβ1–3[SO3-GlcNAcβ1–6]GalNAc | 2.91 |
| 675–1 | NeuAcα2-3Galβ1-3GalNAc | 3.01 |
| 749-PH | GalNAcβ1-4Galβ1-3/4GlcNAcβ1-6Gal | 3.23 |
| 813–1 | Fucα1-2Galβ1–3[SO3-GlcNAcβ1–6]GalNAc | 3.34 |
| 878–1 | GalNAcβ1–4[NeuAc2-3/6]Galβ1-3GalNAc | 3.44 |
| 749–1 | Galβ1–3[Galβ1-3GlcNAcβ1–6]GalNAc | 3.36 |
| 749–2 | Galβ1–3[Galβ1-4GlcNAcβ1–6]GalNAc | 3.48 |
| 829–1 | Galβ1–3[Galβ1-4SO3-GlcNAcβ1–6]GalNAc | 3.65 |
| 829–2 | Galβ1–3[Galβ1-3SO3-GlcNAcβ1–6]GalNAc | |
| 878-PH | GalNAcβ1-4(NeuAcα2–6)Galβ1-3GlcNAc | 3.75 |
| 952-1H | GalNAcβ1-4Galβ1-4GlcNAcβ1-3/6Galβ1-3GalNAc | 3.91 |
| 952-2H | GalNAcβ1-4Galβ1-3GlcNAcβ1-3/6Galβ1-3GalNAc | 3.97 |
| 952-3H | Galβ1–3[GalNAc/GlcNAcβ1-3/4/6Galβ1-4GlcNAcβ1–6]GalNAc | 4.10 |
| 952-4H | Galβ1–3[GalNAc/GlcNAcβ1-3/4/6Galβ1-3GlcNAcβ1–6]GalNAc | 4.14 |
| 1040-P | GalNAcβ1-4(NeuAcα2–6)Galβ1-3/4GlcNAcβ1-6Gal | 4.21 |
| 1040–1 | NeuAcα2-3/6Galβ1–3[Galβ1-3/4GlcNAcβ1–6]GalNAc | 4.35 |
| 1040–2 | Galβ1–3[NeuAcα2-3Galβ1-4GlcNAcβ1–6]GalNAc | 4.52 |
| 1040–3 | Galβ1–3[NeuAcα2-6Galβ1-4GlcNAcβ1–6]GalNAc | 4.58 |
| 1243–1 | GalNAcβ1-4(NeuAcα2–6)Galβ1-3GlcNAcβ1-3/6Galβ1-3GalNAc | 4.81 |
| 1243–2 | Galβ1–3[GalNAc/GlcNAcβ1-3/4(NeuAcα2–6)Galβ1-4GlcNAcβ1–6]GalNAc | 4.97 |
| 1243–3 | NeuAcα2-3/6(GalNAc/GlcNAcβ1-3/4/6)Galβ1–3[Galβ1-4GlcNAcβ1–6]GalNAc | |
| 1243–4 | NeuAcα2-3/6Galβ1-3(GalNAcβ1-4Galβ1-3/4GlcNAcβ1-6GalNAc | 6.18 |
| 1155–1 | GlcNAcβ1-3/6Galβ1–3[GalNAcβ1-4Galβ1-4GlcNAcβ1–6]GalNAc | 5.38 |
| 1389–1 | GalNAcβ1-4(NeuAcα2-3/6)Galβ1–3[Galβ1-4(Fucα1–2)GlcNAcβ1-6GalNAc | 5.62 |
| 1389–2 | [NeuAcα2-3/6] +(GlcNAcβ1-3/6(Fucα1–2)Galβ1–3[Galβ1-4GlcNAcβ1–6]GalNAc | |
| 1446–1 | GlcNAcβ1-3/6Galβ1–3[GalNAcβ1-4(Neu5Acα2–6)Galβ1-3/4GlcNAcβ1–6]GalNAc | 6.10 |
| 1446–2 | GalNAcβ1-4(NeuAcα2–3)Galβ1–3[GalNAcβ1-4Galβ1-4GlcNAcβ1–6]GalNAc | 6.58 |
| 1446–3 | GalNAcβ1-4(NeuAcα2-3/6)Galβ1-3(GalNAcβ1-4Galβ1-3GlcNAcβ1–6)GalNAc | 6.74 |
| 1535–1 | NeuAcα2-3/6Galβ1–3[GalNAcβ1-4(NeuAcα2–6)Galβ1-3/4GlcNAcβ1–6]GalNAc | 6.96 |
| 1608–1 | GalNAcβ1-4(NeuAcα2–6)Galβ1-3/4GlcNAcβ1-3/6Galβ1-3/4GlcNAcβ1-3/6Galβ1-3GalNAc | 7.09 |
| 1811–1 | [GlcNAcβ1–6]GalNAcβ1-4Galβ1-3/4GlcNAcβ1-3/6Galβ1-3/4(NeuAcα2-3/6)GlcNAcβ1-3/6Galβ1-3GalNAc | 7.38 |
| 1738–1 | GalNAcβ1-4(NeuAcα2-3/6)Galβ1–3[GalNAcβ1-4(NeuAcα2–6)Galβ1-3/4GlcNAcβ1–6]GalNAc | 7.51/ 7.59 |
| 2103–1 | GalNAcβ1-4(NeuAcα2–6)Galβ1-3/4GlcNAcβ1-3/6Galβ1–3[GlcNAcβ1-3/6(NeuAcα2-3/6)Galβ1-4GlcNAcβ1–6]GalNAc | 8.14 |
[1] P = peeling, PH = peeling only found in HILIC (not MS), H = glycan found only in HILIC.
Novel structures are highlighted.