FIG. 5.

RV7 vaccination is effective by multiple routes. BALB/c mice were vaccinated with 2 × 10⁵ PFU of RV7, or were mock vaccinated, using the different routes noted on days 0 and 14 of the experiment and then challenged with 10⁴ PFU of sgMCMV i.p. on day 28 of the experiment. For details of vaccination procedures, see Materials and Methods. Four days later, spleens were harvested and the MCMV titer was determined. When spleen titer was not detected by plaque assay, the titer was arbitrarily fixed at 100, the limit of plaque assay sensitivity. Data for mock vaccinations were pooled from four different experiments using s.c., intragastric, and i.p. routes (15 mice in total). Data for RV7 vaccination were pooled from two to three independent experiments and represents 6 to 10 mice per condition. Data are shown as mean log titer ± SEM.