FIG. 7.
Efficiency of RV7 vaccination in mice with various immunodeficiencies. Mice of the indicated strains were vaccinated with 2 × 105 PFU of RV7 s.c., or were mock vaccinated, on days 0 and 14 of the experiment and then challenged with sgMCMV i.p. on day 28 of the experiment. Four days later, spleens were harvested and the MCMV titer was determined. Data were pooled from the following numbers of experiments and numbers of experimental animals: (i) 129, three experiments, 9 to 10 mice per condition, 104 PFU of sgMCMV challenge; (ii) 129 IFNγR−/−, three experiments, 8 to 9 mice per condition, 104 PFU of sgMCMV challenge; (iii) B6.Ig−/−, one experiment, 4 mice per condition, 106 PFU of sgMCMV challenge (similar results were obtained in two additional experiments using a challenge dose of either 5 × 104 PFU [n = 4 mice) or 2 × 105 PFU [n = 4 mice] of sgMCMV); (iv) B6.β2−/−, two experiments, 8 to 9 mice per condition, 106 PFU of sgMCMV challenge (similar results were obtained in a third experiment with a challenge dose of 2 × 105 PFU of sgMCMV). When spleen titer was not detected by plaque assay, the titer was arbitrarily fixed at 100, the limit of plaque assay sensitivity. Data are shown as mean log titer ± SEM.