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. Author manuscript; available in PMC: 2024 Mar 14.
Published in final edited form as: Nat Genet. 2023 Sep 21;55(10):1735–1744. doi: 10.1038/s41588-023-01506-8

Extended Data Fig. 7. Phenotypic analysis of hPSC-derived microglia-like cells under synergistic inhibition of TREM2 enhancer and promoter.

Extended Data Fig. 7.

a, FACS analysis of proliferation for WTC11- and H1-derived microglia-like cells perturbed with synergistic inhibition of TREM2 enhancer and promoter in both control and IFNβ stimulated conditions. b, Representative contour plots of Ki-67 FITC FACS gating strategy. Cells were separated from debris based on the forward scatter area and side scatter area. Two singlet gates were applied using the width and height metrics of the side scatter and forward scatter. Ki-67 FITC signals are shown for all singlets. c, Negative control population using microglia not stained with Ki-67 antibody. d, FACS analysis of phagocytosis capacity for WTC11- and H1-derived microglia-like cells perturbed with synergistic inhibition of TREM2 enhancer and promoter in both control and IFNβ stimulated conditions. e, Representative contour plots of Latex beads-FITC FACS gating strategy. Cells were separated from debris based on the forward scatter area and side scatter area. Two singlet gates were applied using the width and height metrics of the side scatter and forward scatter. Latex beads-FITC signals are shown for all singlets. f, Negative control population using microglia not incubated with Latex beads.