Figure 5.

Characterization of XH001 cells under various stressed conditions by Raman spectroscopy and super-resolution fluorescence imaging; (A) MIC of H₂O₂ against host cells XH001 alone; data were fitted by a dose–response relationship function; (B) Raman spectra of XH001 cells cultured under various stressed conditions, with each spectrum averaged from at least 10 individual plots of three biological replicates; (C) 2D PCA plot of Raman spectra acquired from XH001 cells under different stressed conditions; plots were depicted from principal components 1 (96.71% of the total variation) and 2 (1.65% of the total variation); 95% confidence intervals were represented by ellipses; (D) correlation analysis between cytochrome c (745 cm⁻¹) and saturated lipids (1128 cm⁻¹); Spearman’s correlation coefficient and P-value were labeled in the plots; (E) correlation analysis between Amide I (1660 cm⁻¹) and saturated lipids (1128 cm⁻¹); Spearman’s correlation coefficient and P-value were labeled in the plots; (F) super-resolution fluorescence imaging of BODIPY C_1, C₁₂-labeled XH001 cells cultured inside the anaerobic conditions; scalar bar = 2 μm.