FIG. 6.

Caspase inhibitors CrmA and zVAD-FMK do not significantly inhibit Sindbis virus replication. (A) Recombinant viruses produced in BHK cell supernatants (MOI, 10) during 1-h intervals were collected, and plaque assays were performed in duplicate. Each datum point is the mean of values for three independent wells harvested on the same day, and the results are representative of seven independent experiments. (B) Production of progeny Sindbis virus (AR339) per hour in BHK cells treated with zVAD-FMK or zFA-FMK was determined. The cells were pretreated with 50 μM zVAD-FMK or zFA-FMK for 2 h, and the inhibitors were replenished after 24 h. Each datum point is the mean of values for three independent wells harvested on the same day.