Figure 1.

BG4 retains high-affinity telomeric GQ oligonucleotides of different lengths. (A) Schematic of ELISA experiment. Construct names indicate the number of repeats; e.g. TELO8 is (TTAGGG)₈. Complete sequences shown in Supplementary Tables 1 and 2. Telomeric GQ is depicted in an anti-parallel conformation for simplicity. (B) ELISA binding curves for GQ constructs that vary in TTAGGG repeat number. Binding is represented by absorbance at 450nm vs. BG4 concentration (nM). (C) Apparent dissociation constants (K_d) are calculated from the nonlinear regression curves shown in (B). Data are mean ± SD from 4–5 independent experiments; one-way ANOVA. (D) Schematic SiMPull assay for binding of DNA constructs to BG4 attached to slides via FLAG antibody. (E) Quantification of SiMPull counts of BG4 binding events. Data are mean ± SD from three independent experiments; ordinary one-way ANOVA. Lack of symbol indicates not significant, **P < 0.01, ****P< 0.0001.