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. 2024 Feb 24;11:rbae018. doi: 10.1093/rb/rbae018

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© The Author(s) 2024. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Influence of miR-365-2-5p secreted by M2-exos on MC3T3-E1 osteogenesis. (A) Fluorescence microscopy for GFP expression. (B) MiR-365-2-5p expression in M2 macrophage was confirmed by qRT-PCR. (C) Expression of miR-365-2-5p in cells following co-culture of MC3T3-E1 with M2-exos overexpressing and knocking down miR-365-2-5p by qRT-PCR. (D) ALP staining to detect the impact of M2-exos secreted miR-365-2-5p on MC3T3-E1 osteogenic differentiation (scale bar: 100 µm). (E) ARS staining was employed to observe the influence of miR-365-2-5p secreted by M2-exos on MC3T3-E1 mineralization (scale bar: 100 µm). (F) Influence of miR-365-2-5p secreted by M2-exos on the expression of osteogenesis-related genes (ALP, Runx2 and BMP2) in MC3T3-E1 cells. ^*P<0.05, ^**P<0.01, ^***P<0.001.