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. Author manuscript; available in PMC: 2024 Mar 14.
Published in final edited form as: J Vis Exp. 2024 Jan 5;(203):10.3791/66263. doi: 10.3791/66263

Table of Materials

RESOURCE SOURCE/Reference IDENTIFIER
Animal models
Tg(myl7:nucGFP) transgenic zebrafish Burns Lab in Boston Children’s Hospital ZDB-TGCONSTRCT-070117-49
Software and algorithms
MATLAB The MathWorks Inc. R2023a
LabVIEW National Instruments Corporation 2017 SP1
HCImage Live Hamamatsu Photonics 4.6.1.2
Python The Python Software Foundation 3.9.0
Fiji-ImageJ Schneider et al.18 1.54f
3DeeCellTracker Chentao Wen et al.15 v0.5.2
Unity Unity Software Inc. 2020.3.2f1
Amira Thermo Fisher Scientific 2021.2
3D Slicer Andriy Fedorov et al.17 5.2.1
ITK SNAP Paul A Yushkevich et al.16 4
Light-sheet system
Cylindrical lens Thorlabs ACY254-050-A
4X Illumination objective Nikon MRH00045
20X Detection objective Olympus 1-U2M585
sCMOS camera Hamamatsu C13440-20CU
Motorized XYZ stage Thorlabs PT3/M-Z8
Two-axis tilt stage Thorlabs GN2/M
Rotation stepper motor Pololu 1474
Fluorescent beads Spherotech FP-0556-2
473nm DPSS Laser Laserglow R471003GX
532nm DPSS laser Laserglow R531003FX
Microinjector and vacuum pump
Microinjector WPI PV850
Vacuum pump Welch 2522B-01
Pre-Pulled Glass Pipettes WPI TIP10LT
Capillary tip for gel loading Bio-Rad 2239912
Virtual reality hardware
VR headset Meta Quest 2
30mg/L PTU solution
PTU Sigma-Aldrich P7629
1X E3 working solution - -
1% Agarose
Low-melt agarose Thermo Fisher 16520050
Deionized water - -
10g/L Tricaine stock solution
Tricaine Syndel SYNC-M-GR-US02
Deionized water - -
Sodium bicarbonate Sigma-Aldrich S6014
150mg/L Tricaine working solution
10g/L Tricaine stock solution - -
Deionized water - -
60X E3 stock solution Lab Animal Resource Center (LARC), The University of Texas at Dallas
Sodium Chloride NaCl
Potassium Chloride KCL
Calcium Chloride Dihydrate CaCL2 x 2H2O
Magnesium Sulfate Heptahydrate MgSO4 x 7H2O
RO Water -
1X E3 working solution
60X E3 stock solution -
RO Water -
1% Methylene Blue (optional) C16H18ClN3S