Figure 3 – Peripheral G-CSF and neutrophilic cells return to baseline after primary tumor resection.

A, illustration of the experiment designed to measure differences in the blood and spleens of tumor bearing or tumor resected mice.
B, a connected-line dot plot shows plasma G-CSF concentrations in tumor bearing or tumor resected mice. The black arrow indicates the time of primary tumor resection (day 20). The G-CSF concentration in mice without tumors is shown as a dotted line.
Connected-line dot plots show the quantification of Ly6G+ cells as a percentage of live total leukocytes in the spleens (C) or blood (D) of tumor bearing or tumor resected mice (n=10 mice total from two independent experiments for each condition and each timepoint). The black arrow indicates the time of primary tumor resection (day 20). The percentage of Ly6G+ cells in mice without tumors is shown as a dotted line in each plot.
For B, C and D, *, P<0.05; **, P<0.01, ***, P<0.001; significance was determined using multiple unpaired t-tests with each timepoint considered independently.