Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rat Anti-mouse CD45-PercP (30-F11) | BioLegend | Cat# 103130; RRID AB_893339 |
| Rat Anti-mouse CD3-BUV805 (17A2) | BD Biosciences | Cat# 569192; RRID AB_2871285 |
| Rat Anti-mouse CD4-BUV496 (GK1.5) | BD Biosciences | Cat# 612952; RRID AB_2813886 |
| Rat Anti-mouse CD8-BB515 (53–6.7) | BD Biosciences | Cat# 564422; RRID AB_2738801 |
| Mouse Anti-Mouse Tbet-BUV421 (4B10) | BioLegend | Cat# 644816; RRID AB_10959653 |
| Rat Anti-mouse IL-2-AF700 (JES6-5H4) | BioLegend | Cat# 503818; RRID AB_528931 |
| Rat Anti-mouse IFNγ-BUV737 (XMG1.2) | BD Biosciences | Cat# 612769; RRID AB_2870098 |
| Rat Anti-mouse Foxp3-AF532 (FJK-16s) | ThermoFischer | Cat# 58-5773-82; RRID AB_11218870 |
| Hamster Anti-mouse CD279(PD-1)-BUV615 (J43) | BD Biosciences | Cat# 752299; RRID AB_2875816 |
| Purified anti-mouse CD16/CD32 FC Shield (clone 2.4G2) | Tonbo Biosciences | Cat#: 70-0161-M001, RRID: N/A |
| Rabbit Anti-mouse CGRP | Invitrogen | Cat# PA5-114929; RRID AB_2899565 |
| Chicken Anti-mouse NF-H | Invitrogen | Cat# PA1-10002; RRID AB_1077155 |
| Rabbit Anti-mouse TRPV1 | Alomone Labs | Cat# ACC-030-200UL |
| Mouse Anti-mouse ChAT | Invitrogen | Cat# MA5-31383; RRID AB_2787020 |
| Goat Anti-mouse IgG (H+L) highly cross-adsorbed AF Plus 488 | Invitrogen | Cat# A-11055; RRID AB_2534102 |
| Goat Anti-rabbit IgG (H+L) cross-adsorbed AF Plus 647 | Invitrogen | Cat# A32733; RRID AB_2633282 |
| Goat Anti-chicken IgY (H+L) AF 647 | Invitrogen | Cat# A-21449; RRID AB_2535866 |
| InVivoMab Anti-mouse CD3 | BioXCell | Cat# BE0002 |
| InVivoMab Anti-mouse CD28 | BioXCell | Cat# BE0015-1 |
| Biological samples | ||
| Human HNSCC – isolated nerves | University of California Los Angeles | Collected per COMIRB-11-002858 |
| Human HNSCC – tumor tissue | University of Colorado | Clinical Trials.gov NCT03635164 |
| Chemicals, peptides, and recombinant proteins | ||
| Fetal Bovine Serum | Gibco | Cat# 10437028 |
| RPMI 160 Medium | Gibco | Cat# 11875-093 |
| DMEM/F-12 | Gibco | Cat# 10565042 |
| Primocin | InvivoGen | Cat# ant-pm-05 |
| Fungin | InvivoGen | Cat# ant-fn-1 |
| BIBN4096 | Tocris | Cat# 4561 |
| pLVX-puro-cOVA-IRES-BFP plasmid | Addgene | Plasmid #135074 |
| psPAX2 plasmid | Addgene | Plasmid #12260 |
| pMD2.G plasmid | Addgene | Plasmid #12259 |
| Puromycin | Invivogen | Cat# ant-pr-1 |
| Matrigel | BD Biosciences | Cat# 05850 |
| Brilliant Stain Buffer | BD Biosciences | Cat# 563794 |
| Trypsin Inhibitor, Soybean Purified, AOF | Worthington Biochemical Corporation | Cat# LS003571 |
| Collagenase Type III | Worthington Biochemical Corporation | Cat# LS004182 |
| eBioscience 1X RBC Lysis Buffer | Invitrogen | Ref# 00-4333-57 |
| eBioscience FOXP3/Transcription Factor Fixation/Permeabilization | Invitrogen | Ref# 00-5521-00 |
| Live/Dead Aqua Fixable Viability Dye | Invitrogen | Cat#: L34966 |
| Calcein, AM | Invitrogen | Cat#: C3099 |
| DAPI Fluoromount-G® | SouthernBiotech | Cat# 0100-20 |
| CryoStor CS10 | STEMCELL | Cat# 07931 |
| Xylene | Fischer Scientific | REF#X3P-1GAL |
| Vector Unmasking Solution | Vector Laboratories | REF#H-3300 |
| TWEEN | Sigma-Aldrich | LOT#SLCB6677 |
| Bovine Albumin Serum Fraction V | Sigma-Aldrich | REF#03116956001 |
| Critical commercial assays | ||
| EasySep Mouse CD8+ T cell isolation kit | StemCell Technologies | Cat#: 19853 |
| Mouse CGRP-I ELISA EIA Kit | RayBiotech | Code: EIAM-CGRP-1 |
| CGRP | Phenix Pharmaceuticals | Cat# 015-09 |
| Mouse IFNγ Single-Color ELISPOT | ImmunoSpot | |
| Deposited data | ||
| Human RNA Sequencing Data | Darragh et al. https://doi.org/10.1038/s43018-022-00450-6 |
GEO: GSE210287 |
| Human Nerve Proteomics Data | This paper | Supplemental File. |
| Experimental models: Cell lines | ||
| MOC2 | Provided by R. Uppaluri | RRID: CVCL_ZD38 |
| LY2 | Provided by Nadarajah Vigneswaran | RRID: CVCL_Z594 |
| P029 | Provided by X.J. Wang | N/A |
| HEK293-FT cells | Provided by Molishree Joshi | |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J | Jackson Laboratories | Strain Code: 000664 |
| Mouse: Balb/C | Charles River | Strain Code: 028 |
| Mouse: TRPV1 KO | Jackson Laboratories | Strian Code: 003770 |
| Software and algorithms | ||
| Flowjo (v10.8.1) | BD Bioscience | https://www.flowjo.com |
| Grahpad Prism (v9.1.0) | Dotmatics | https://www.graphpad.com |
| Biorender | Biorender.com | |
| R (v.4.2.3 and v. 4.2.1) | R Foundation for Statistical Computing | |
| clusterProfiler (v.4.6.2 and 4.4.4) | Wu, T. et al. clusterProfiler 4.0: A universal enrichment tool for interpreting omics data. Innovation (Camb) 2, 100141 (2021). | |
| ComplexHeatmap (v.2.14.0 and v.2.12.1) | Gu, Z., Eils, R. & Schlesner, M. Complex heatmaps reveal patterns and correlations in multidimensional genomic data. Bioinformatics (Oxford, England) 32, 2847-2849 (2016). | |
| Metaboanalyst 5.01 | https://www.metaboanalyst.ca/ | |