REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-RFP	Rockland	Cat#600-401-379; RRID:AB_2209751
Chicken anti-GFP	abcam	Cat# ab13970; RRID:AB_300798
Rat anti-NCAD	Developmental Studies Hybridoma Bank	Cat#DN-Ex #8; RRID:AB_528121
Rabbit anti-histamine	Immunostar	Cat#22939; RRID:AB_572245
Rabbit anti-hemaglutinin	Cell Signaling Technology	Cat#3724; RRID:AB_1549585
Mouse anti-V5-Alexa 555	Bio-Rad	Cat# MCA1360D550GA; RRID:AB_2687576
Rabbit anti-GABA	Sigma	Cat#A2052; RRID:AB_477652
Rabbit anti-serotonin	Immunostar	Cat#20080; RRID:AB_572263
Rabbit anti-tyrosine hydroxylase	Immunostar	Cat#22941; RRID:AB_572268
Mouse anti-Bruchpilot (nc82)	Developmental Studies Hybridoma Bank	Cat#nc82; RRID:AB_2314866
Donkey anti-chicken AlexaFluor 488	Jackson ImmunoResearch Laboratories	Cat#703-545-155; RRID:AB_2340375
Donkey anti-rabbit AlexaFluor 546	Invitrogen	Cat#A-10040; RRID:AB_2534016
Donkey anti-rat AlexaFluor 647	Abcam	Cat#ab150155; RRID:AB_2813835
Donkey anti-mouse Dylight 649	Jackson ImmunoResearch Laboratories	Cat#715-495-150
Experimental models: Organisms/strains
D. melanogaster: R48H10-GAL4	Jenett et al.109	BDSC #50395; RRID:BDSC_50395
D. melanogaster: UAS-Cbeta\DT.I	Han et al.24	BDSC #25039; RRID:BDSC_25039
D. melanogaster: hsFlp;;MCFO	Nern et al.25	BDSC #64085; RRID:BDSC_64085
D. melanogaster: 40XUAS-IVS-mCD8::GFP	Pfeiffer et al.110	BDSC #32195; RRID:BDSC_32195
D. melanogaster: pJFRC59-13XLexAop2-IVS-myr::GFP	Pfeiffer et al.111	Janelia Fly store; 1117286
D. melanogaster: R84G04-Gal4	Jenett et al.109	BDSC #40403; RRID:BDSC_40403
D. melanogaster: R84G04-LexA (Chr. 3)	Pfeiffer et al.110; Mobilized derivative of RRID:BDSC_54987	BDSC #54987; RRID:BDSC_54987
D. melanogaster: R17F12-p65AD	Dionne et al.112	BDSC #68845; RRID:BDSC_68845
D. melanogaster: VT049652-Gal4.DBD	Tirian et al.113	BDSC #74970; RRID:BDSC_74970
D. melanogaster: VT025938-p65AD	Tirian et al.113	BDSC #71314; RRID:BDSC_71314
D. melanogaster: VT040583-GAL4.DBD	Tirian et al.113	BDSC #71800; RRID:BDSC_71800
D. melanogaster: UAS-DenMark, UAS-syt.eGFP	Nicolaï et al.32	BDSC #33065; RRID:BDSC_33065
D. melanogaster: VT039465-p65AD;VT023750-Gal4.DBD	Namiki et al.33	Janelia line SS02625; BDSC #75974; RRID:BDSC_75974
D. melanogaster: R24A03-p65AD; R74C01-Gal4.DBD	Namiki et al.33	Janelia line SS01062; BDSC #86738; RRID:BDSC_86738
D. melanogaster: VT056359-p65AD; VT002021-Gal4.DBD	This paper	Janelia line SS96091; BDSC #87313; RRID:BDSC_87313
D. melanogaster: 10XUAS-IVS-mCD8::RFP}attP18,P{y[+t7.7]w[+mC]=13XLexAop2-mCD8::GFP}su(Hw)attP8	Pfeiffer et al.110	BDSC #32229; RRID:BDSC_32229
D. melanogaster: pJFRC28-10XUAS-IVS-GFP-p10 in attP2	Pfeiffer et al.111	Janelia Fly store #1116592
D. melanogaster: ChAT-Trojan-LexA	Diao et al.40	BDSC #60317; RRID:BDSC_60317
D. melanogaster: vGlut-Trojan-LexA	Diao et al.40	BDSC #60314; RRID:BDSC_60314
D. melanogaster: GAD1-Trojan-LexA	Diao et al.40	BDSC #60324; RRID:BDSC_60314
D. melanogaster: 20XUAS-IVS-Syn21-jGCaMP7f-p10 in su(Hw)attP5	Dana et al.41	BDSC #80906; RRID:BDSC_80906
D. melanogaster: 20XUAS-IVS-Syn21-jGCaMP7f-p10 in VK00005	Dana et al.41	BDSC #79031; RRID:BDSC_79031
D. melanogaster: 20XUAS-IVS-Syn21-jGCaMP7b-p10 in su(Hw)attP5	Dana et al.41	BDSC #80907; RRID:BDSC_80907
D. melanogaster: 20XUAS-IVS-Syn21-jGCaMP7b-p10 in VK00005	Dana et al.41	BDSC #79029; RRID:BDSC_79029
D. melanogaster: 13XLexAop2-IVS-Syn21-jGCaMP7f in VK00005	Dana et al.41	BDSC #80914; RRID:BDSC_80914
D. melanogaster: 13xLexAop2-IVS-Syn21-jGCaMP7f in su(Hw)attP5	Dana et al.41	Janelia Fly Store #3032633
D. melanogaster: 20xUAS-CsChrimson-tdTomato-trafficked in su(Hw)attP1	Klapoetke et al.42	Janelia Fly Store #3015695
Software and algorithms
R version 4.1.1	The R Foundation	https://www.r-project.org/
Fiji ver 2.3.0/1.53f51	Schindelin et al.107	https://fiji.sc/
Scanimage version 2016	Pologruto et al.106	https://www.mbfbioscience.com/products/scanimage/
μManager	Edelstein et al.105	https://micro-manager.org/
Code for connectomics and calcium imaging analyses	This paper; GitHub; Zenodo	https://doi.org/10.5281/zenodo.10557545