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. 2024 Jan 23;134(6):e171166. doi: 10.1172/JCI171166

Figure 9. ZMYND8 is a direct NRF2 target gene.

Figure 9

(A) Correlation analysis between ZMYND8 and NFE2L2 mRNAs in human breast tumors from TCGA cohort. (B and C) Analysis of ZMYND8 mRNA in MDA-MB-231-SC, (B) and MCF-7-SC, (C) NRF2 KO1, and NRF2 KO2 mammospheres (n = 3–4). (D) Analysis of ZMYND8 and NRF2 protein levels in MDA-MB-231-SC and MCF-7-SC, NRF2 KO1, and NRF2 KO2 cells after MG132 (10 μM) treatment (n = 3). (E) NRF2 enrichment at the ZMYND8 promoter in MDA-MB-231 mammospheres (top). The ZMYND8 ARE sequence is shown at the bottom. Rep, replicate; AREmu, ARE mutant. (F and G) Luciferase reporter assay in HEK293T cells cotransfected with indicated plasmids. The firefly/Renilla luciferase activity is quantified (F) (n = 4). Analysis of NRF2 protein levels (G). Data represent mean ± SEM. P value was determined by using 1-way ANOVA corrected with Dunnett’s test (B and C) and 2-way ANOVA corrected with Tukey’s test (F). ***P < 0.001; ****P < 0.0001.