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. 2024 Feb 6;134(6):e174847. doi: 10.1172/JCI174847

Figure 4. Paclitaxel treatment induces tactile and thermal hypersensitivity and potentiates glutamate release from primary afferent nerve terminals via METTL14.

Figure 4

(A) Illustration of the experimental design. (B–D) Mechanical allodynia, thermal hyperalgesia, and cold allodynia of saline- or PCX-treated rats after siMettl14 or scrambled siRNA treatment measured by the von Frey test (B), hot plate test (C), and acetone test (D), respectively (at least 7 rats per group; *PCX+siMettl14- vs. PCX+siNC-treated rats, #PCX+siNC- vs. SAL+siNC-treated rats, $PCX- vs. SAL-treated rats). siNC, negative control RNA. (E) Schematic of the METTL14 therapeutic role evaluation. (F–H) Mechanical allodynia, thermal hyperalgesia, and cold allodynia were measured following PCX treatment and siRNA injection (7 rats per group, 2-way ANOVA followed by Tukey’s post hoc test; *PCX+siMettl14 vs. PCX+siNC). (I and J) Representative recording traces and quantification show the baseline control and the effect of bath application of AP5 on monosynaptic EPSCs of a lamina II neuron evoked from the dorsal root from saline- or PCX-pretreated rats after siMettl14 or scrambled siRNA treatment. (K and L) Representative recording traces and quantification show the baseline control and the effect of bath application of AP5 on the EPSCs evoked by a pair of pulses from saline- or PCX-pretreated rats after siMettl14 or scrambled siRNA treatment (at least 3 rats, 8 neurons per group; *compared with baseline between each group, #compared with the respective baseline). *P < 0.05, **P < 0. 01, ***P < 0.001, ****P < 0. 0001; ##P < 0.01, ###P < 0.001, ####P < 0.0001; $P < 0.01, $$$P < 0.001, $$$$P < 0.0001. Two-way ANOVA followed by Tukey’s post hoc test.