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. 2024 Mar 15;134(6):e175158. doi: 10.1172/JCI175158

Figure 8. FUS nuclear translocation regulates the expression of fibrotic markers in CCl4-induced liver fibrosis.

Figure 8

(A) Nuclear fractions (50 μg/lane) of livers from uninjured (vehicle) mice and mice treated for 6 weeks with CCl4 alone or in combination with CP-mutFUS-NLS or CP-FUS-NLS peptide were analyzed by Western blot for FUS levels. Histone H3 and GAPDH were used to verify the purity of nuclear fractions. (B) Nonnuclear fractions (50 μg/lane) of livers from the mice described in A were analyzed by Western blot for levels of collagens I and IV, desmin, and TIMP1. Histone H3 and GAPDH were used to verify the purity of nonnuclear fractions. (C–G) Immunoreactive bands were quantified by densitometric analysis, and values were expressed as ratios relative to histone H3 for nuclear proteins or GAPDH for nonnuclear proteins. Values are the mean ± SD, and symbols represent individual livers (n = 7 vehicle, n = 8–10 CCl4, n = 9–10 CCl4+CP-mutFUS-NLS, n = 10 CCl4+CP-FUS-NLS). Statistical analysis: 1-way ANOVA followed by Dunnett’s multiple-comparison test (C–G).