TABLE 2.
Binding of Bel-1 and Tas to minimal DNA target sites in yeast cellsa
| Promoter | β-Gal activity with plasmid
|
||
|---|---|---|---|
| Control | +Bel-1 | +Tas | |
| pJLB | ≤5 | ≤5 | ≤5 |
| HFV Int. Pr. (1× WT+) | ≤5 | 86 | 11 |
| HFV Int. Pr. (1× WT−) | ≤5 | 167 | 17 |
| HFV Int. Pr. (2× WT+) | ≤5 | 701 | 120 |
| HFV Int. Pr. (2× WT−) | ≤5 | 1,262 | 54 |
| HFV Int. Pr. (2× M+) | 22 | 17 | 48 |
| HFV Int. Pr. (2× M−) | 22 | 23 | 31 |
| SFV-1 Int. Pr. (1× WT+) | ≤5 | ≤5 | 2,870 |
| SFV-1 Int. Pr. (1× WT−) | ≤5 | ≤5 | 714 |
a
Wild-type (WT) or mutant (M) forms of the minimal 25-bp HFV or SFV-1 internal promoter (Int. Pr.) sequence shown in Fig. 5 were inserted in one (1×) or two (2×) copies into the lacZ-based yeast indicator plasmid pJLB. Orientation is indicated by a plus (sense) or minus (antisense) sign. These reporter plasmids were introduced into yeast cells together with single-copy plasmids expressing Bel-1 or Tas or a similar control plasmid. Induced β-Gal activity was measured after selection for cotransformants and is given in mOD/milliliter. These data are representative of three independent experiments.