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. 2023 May 22;42(3):498–509. doi: 10.1038/s41587-023-01791-y

Fig. 2. Broadened editing to 5′-GAT-3′ by introducing mutations to MutH.

Fig. 2

a, Crystal structure of key amino acids of MutH interacting with unmethylated 5′-GATC-3′ (PDB: 2AOQ). b, Editing efficiency of MutH mutants (including K48A, E91A, F94A, R184A, Y212S and double mutation of E91A and F94A) combined with TadA8e-V106W at the 5′-GATC-3′ position. c–e, The editing efficiencies of target regions at the 5′-GATA-3′ (c), 5′-GATG-3′ (d) and 5′-GATT-3′ positions (e) with different mitoABEMutH* orientations and distances. In b–e, the mean values from n = 3 biologically independent replicates are shown. f,g, The designable targeting range of TALE–MutH (f) and TALE–MutH* (g) in human mitochondria. The numbers 0, 2, 4 and 6 indicate the frequency of MutH and MutH* recognition sequences within a 40-bp region. In b, c, d and e, the blue rounded rectangle represents TadA8e-V106W, the orange rounded rectangle represents the MutH mutant and the gray rectangle represents TALE. The borders of rounded rectangles of different colors (blue and green) represent TALE–MutH* designed to nick different strands. WT, wild type.