Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 May 22;42(3):498–509. doi: 10.1038/s41587-023-01791-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a, Overview of circRNA-encoded mitoABE^MutH-transfected cells. b,c, circRNAs of two mitoABE^MutH orientations were transfected into different cell lines to achieve strand-biased editing, left TALE–MutH with right TALE–TadA8e-V106W (b), left TALE–TadA8e-V106W with right TALE–MutH (c). Genomic DNA was collected 2 days posttransfection. d, Overview of circRNA-encoded mitoABE^Nt.BspD6I(C)-transfected HEK293T cells and genomic DNA collected 2 days posttransfection. e, The editing efficiencies of circRNA-encoded mitoABE^Nt.BspD6I(C) targeted the start codon of MT-ND4. f, The ATP levels of cells transfected with circRNA-encoded mitoABE^Nt.BspD6I(C) targeted the start codon of MT-ND4. Student’s t test, P = 2.71 × 10⁻⁵. g, Oxygen consumption rate (OCR) in HEK293T cells treated with circRNA-encoded mitoABE^Nt.BspD6I(C) targeted the start codon of MT-ND4 for 2 days. h, Overview of circRNA-encoded mitoABE^Nt.BspD6I(C)-transfected LHON disease cells GM10742, with genomic DNA was collected 3 days posttransfection. i, The editing efficiency of mitoABE^Nt.BspD6I(C) corrected the 11778G>A mutation of LHON disease cell GM10742. j, The ATP levels of cells transfected with circRNA-encoded mitoABE^Nt.BspD6I(C) targeting the 11778G>A mutation of LHON disease cell GM10742. Student’s t test, P = 6.98 × 10⁻⁵. k, OCR of the LHON disease cell line GM10742 treated with circRNA-encoded mitoABE^Nt.BspD6I(C) targeting the 11778G>A mutation for 2 days. l, Types of mitochondrial diseases (MITOMAP) and the proportion of diseases that can theoretically be treated by mitoBEs. For b, c, f, g, j and k, the data are presented as mean values ±s.d. of n ≥ 3 independent biological replicates. For e and i, the mean values from n = 3 biologically independent replicates are shown. For g,k, FCCP represents carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone.