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. 2023 May 22;42(3):498–509. doi: 10.1038/s41587-023-01791-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a, Different orientations of mitoABE^MutH when the 3 bp distance from 5’-GATC-3’ and the adenines on different strands are edited at MT-RNR2 site 1, MT-ND1 site 1 and MT-ND4 site 1. Top, Left-TALE-MutH and Right-TALE- TadA8e(V106W). Bottom, Left-TALE-TadA8e(V106W) and Right-TALE- MutH. b, Different orientations of mitoABE^MutH when the 5 or 6 bp distance from 5’-GATC-3’ and the adenines on different strands are edited at MT-RNR2 site 2 and MT-ND4 site 1. Top, Left-TALE-TadA8e(V106W) and Right-TALE-MutH. Bottom, Left-TALE-MutH and Right-TALE-TadA8e(V106W). For a and b, data are presented as mean values ± s.d. of n = 3 independent biological replicates. In a and b, the blue rounded rectangle represents TadA8e(V106W), the purple rounded rectangle represents nickase, and the gray rectangle represents TALE. c and d, Editing efficiency of mitoABE^MutH with MutH and TadA8e(V106W) at different distances from 5’-GATC-3’ at MT-RNR2 site 1 (c) and MT-ND4 (d). For c and d, the mean values from n = 3 biologically independent replicates are shown.