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. 2023 Dec 22;31(3):410–419. doi: 10.1038/s41417-023-00711-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A The efficiency of SPHK1 overexpression and knockdown was measured by western blot (left). The changes in the expression of TRAF6 level following SPHK1 overexpressed or down-regulated in HT29 or RKO cells identified by western blot (right). B Immunofluorescence assay revealed that SPHK1 regulated the level of TRAF6. Scale bar, 100 µm. Data were presented by mean with SD (n ≥ 3). *P < 0.05, **P < 0.01.