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. 2023 Dec 22;31(3):410–419. doi: 10.1038/s41417-023-00711-1

Fig. 7. The inhibitory effect of SPHK1 knockdown on CRC cells proliferation and metastasis was rescued by the overexpression of TRAF6.

Fig. 7

A Dual-gene transfection cells of SPHK1(-)-NC-RKO and SPHK1(-)-TRAF6(+)-RKO were successfully constructed, and evaluated by western blot. B Overexpression of TRAF6 enhanced SPHK1 knockdown induced suppression of CRC cell growth, evaluated by CCK8 and colony formation assays. C Overexpression of TRAF6 enhanced SPHK1 knockdown induced suppression of CRC cell metastasis, evaluated by wound healing assay and transwell. Scale bar, 200 µm. D Overexpression of TRAF6 rescued the expression of autophagy and EMT proteins in SPHK1(-)-RKO cells, evaluated by Western blot. Data were presented by mean with SD (n ≥ 3). *P < 0.05, **P < 0.01.