Fig. 4. Siglec-15⁺ macrophages were negatively associated with inflamed immunophenotype in PD-L1⁻ patients.

Among Mφ-Siglec-15 and PD-L1 patterns in non-metastasis LUAD patients, box plot showed the density of A CD4⁺T cells, B CD8⁺T cells, C CD68⁺TAMs and D CD4⁺FoxP3⁺Tregs. The bar indicates mean with 95% CI. E Representative slide images of CD8⁺T cell immunophenotypes with the percentage of patients per phenotype and representative multiplex IF images of immune effector cells at tumor and stroma area of each phenotype. Scale bar, 50 μm. F The proportions of immunophenotypes among expression patterns of Mφ-Siglec-15 and PD-L1. G Comparison of density of Siglec-15⁺CD68⁺TAMs between Mφ-Siglec-15⁻PD-L1⁻ and Mφ-Siglec-15⁺PD-L1⁻LUAD patients. The bar indicates mean with 95% CI.