TABLE 2.
Cellular cloning of the mNDK-infected HeLa and SW480 populations: coculture analysis of cell clone entry phenotype
| Syncytium formationa
|
No. of clonesb
|
||
|---|---|---|---|
| HeLaCD4LTRLacZ | HeLaLTRLacZ | HeLa | SW480 |
| − | − | 45 | 4 |
| + | ± | 28 | 11 |
| ++ | ± | 15 | 0 |
| +++ | ++ | 22c | 0 |
| ++++ | +++ | 6c | 1 |
Syncytia were scored as follows: −, <5; ±, 5 to 10; +, 10 to 50; ++, 50 to 200; +++, 200 to 1,000; and ++++, >1,000.
Independent clones were recovered by using glass cylinders.
Twenty-eight clones, from which clones 2, 14, and 48 were selected.