FIGURE 4.

Effect of TID1 overexpression on frataxin protein levels. HEK293 cells transfected with frataxin and TID1 plasmid DNAs were lysed and subjected to Western blotting with the indicated antibodies. The amount of immunoreactivity in the lysates was quantified as a percentage of vector control. Representative blots and bar graphs demonstrate increased frataxin precursor and decreased intermediate and mature frataxin following TID1L or TID1S overexpression (A,B) (n = 8). TID1L H121Q and TID1S H121Q had similar effects as wildtype TID1L and TID1S (A,B) (n = 6), respectively. No change was found in GRP75 levels upon TID1L or TID1S overexpression (C). ∗p < 0.05, ∗∗p < 0.01. Data were shown as mean ± SE.