Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Mar 1;6:1346781. doi: 10.3389/fgeed.2024.1346781

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2024 Bijlani, Pang, Bugga, Rangasamy, Narayanan and Chatterjee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Restoration of MeCP2 expression in edited male hemizygous r.378_384del fibroblasts. Intranuclear expression of MeCP2 is shown in male hemizygous fibroblasts after transduction with the AAVHSC7-226 editing vector. (A–C) MECP2 staining in untransduced and transduced r.378_384del fibroblasts. (A) Unedited cells show no MeCP2 expression due to a deletion in C-Ter. (B,C) MeCP2 expression is observed in nuclei of r.378_384del fibroblasts 48 h after transduction with the AAVHSC7-226 editing vector. (D) No Venus expression is observed in untransduced cells. (E, F) show Venus expression in the cytoplasm after overlay of staining with an anti-GFP antibody. Also shown in (D–F) is the overlay with DAPI staining. Yellow arrows: examples of cells expressing both MeCP2 and Venus.