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. 2024 Feb 5;33(2):177–189. doi: 10.1007/s10646-024-02737-z

Table 2.

Results from GLMMs examining differences in log-transformed mass (g) between exposure treatments and the control (intercept) in the chronic exposure study of Rana pipiens tadpoles exposed to PFBA and PFHxA

n ß coef SE df t-value p-value Variance SD
PFBA
Model 3: logMass ~ Treatment + SVL + Stage + Stage ^ 2 + Stage ^ 3 + (1 | Tank), n = 247
 Fixed Effects (Intercept - Control) 50 9.273 1.869 230.2 4.963 <0.001
0.1 µg/L 40 0.042 0.018 24.8 2.374 0.026
1 µg/L 38 0.049 0.018 25.4 2.747 0.011
10 µg/L 39 0.043 0.018 24.8 2.424 0.023
100 µg/L 40 0.037 0.018 24.6 2.068 0.049
1000 µg/L 40 0.021 0.018 24.7 1.151 0.261
SVL 0.051 0.002 237.7 25.636 <0.001
Stage −0.996 0.158 230.2 −6.303 <0.001
Stage^2 0.031 0.004 230.3 7.077 <0.001
Stage^3 −3.21 × 10−4 4.108 × 10−5 230.4 −7.815 <0.001
 Random Effects Tank < 0.001 0.022
Residual 0.002 0.045
PFHxA
Model 4: logMass ~ Treatment + SVL + Stage + Stage ^ 2 + Stage ^ 3 + (1 | Tank), n = 246
 Fixed Effects (Intercept - Control) 50 5.830 1.450 231.4 4.021 <0.001
0.1 µg/L 40 0.062 0.022 25.1 2.833 0.009
1 µg/L 39 0.056 0.022 24.9 2.568 0.017
10 µg/L 38 0.052 0.022 25.8 2.366 0.026
100 µg/L 40 0.024 0.022 24.7 1.102 0.281
1000 µg/L 39 0.039 0.022 25.2 1.774 0.088
SVL 0.050 0.002 230.0 24.354 <0.001
Stage −0.702 0.125 231.1 −5.628 <0.001
Stage^2 0.023 0.004 230.9 6.493 <0.001
Stage^3 −2.426 × 10−4 3.336 × 10−5 230.8 −7.273 <0.001
 Random Effects Tank < 0.001 0.028
Residual 0.003 0.050

Treatment, snout-to-vent length (SVL) (mm), and Gosner stage of development (Stage) were included as fixed effects, and tank was included as a random effect. Significant results are in bold