Table 2.
Results from GLMMs examining differences in log-transformed mass (g) between exposure treatments and the control (intercept) in the chronic exposure study of Rana pipiens tadpoles exposed to PFBA and PFHxA
| n | ß coef | SE | df | t-value | p-value | Variance | SD | ||
|---|---|---|---|---|---|---|---|---|---|
| PFBA | |||||||||
| Model 3: logMass ~ Treatment + SVL + Stage + Stage ^ 2 + Stage ^ 3 + (1 | Tank), n = 247 | |||||||||
| Fixed Effects | (Intercept - Control) | 50 | 9.273 | 1.869 | 230.2 | 4.963 | < 0.001 | ||
| 0.1 µg/L | 40 | 0.042 | 0.018 | 24.8 | 2.374 | 0.026 | |||
| 1 µg/L | 38 | 0.049 | 0.018 | 25.4 | 2.747 | 0.011 | |||
| 10 µg/L | 39 | 0.043 | 0.018 | 24.8 | 2.424 | 0.023 | |||
| 100 µg/L | 40 | 0.037 | 0.018 | 24.6 | 2.068 | 0.049 | |||
| 1000 µg/L | 40 | 0.021 | 0.018 | 24.7 | 1.151 | 0.261 | |||
| SVL | 0.051 | 0.002 | 237.7 | 25.636 | <0.001 | ||||
| Stage | −0.996 | 0.158 | 230.2 | −6.303 | <0.001 | ||||
| Stage^2 | 0.031 | 0.004 | 230.3 | 7.077 | <0.001 | ||||
| Stage^3 | −3.21 × 10−4 | 4.108 × 10−5 | 230.4 | −7.815 | <0.001 | ||||
| Random Effects | Tank | < 0.001 | 0.022 | ||||||
| Residual | 0.002 | 0.045 | |||||||
| PFHxA | |||||||||
| Model 4: logMass ~ Treatment + SVL + Stage + Stage ^ 2 + Stage ^ 3 + (1 | Tank), n = 246 | |||||||||
| Fixed Effects | (Intercept - Control) | 50 | 5.830 | 1.450 | 231.4 | 4.021 | < 0.001 | ||
| 0.1 µg/L | 40 | 0.062 | 0.022 | 25.1 | 2.833 | 0.009 | |||
| 1 µg/L | 39 | 0.056 | 0.022 | 24.9 | 2.568 | 0.017 | |||
| 10 µg/L | 38 | 0.052 | 0.022 | 25.8 | 2.366 | 0.026 | |||
| 100 µg/L | 40 | 0.024 | 0.022 | 24.7 | 1.102 | 0.281 | |||
| 1000 µg/L | 39 | 0.039 | 0.022 | 25.2 | 1.774 | 0.088 | |||
| SVL | 0.050 | 0.002 | 230.0 | 24.354 | < 0.001 | ||||
| Stage | −0.702 | 0.125 | 231.1 | −5.628 | < 0.001 | ||||
| Stage^2 | 0.023 | 0.004 | 230.9 | 6.493 | < 0.001 | ||||
| Stage^3 | −2.426 × 10−4 | 3.336 × 10−5 | 230.8 | −7.273 | < 0.001 | ||||
| Random Effects | Tank | < 0.001 | 0.028 | ||||||
| Residual | 0.003 | 0.050 | |||||||
Treatment, snout-to-vent length (SVL) (mm), and Gosner stage of development (Stage) were included as fixed effects, and tank was included as a random effect. Significant results are in bold