Table 1.
Candidate reference genes ranked by geNorm. α-EF: α-Elongation factor; His: Histone; UBC: Ubiquitin conjugating enzyme; 18S: 18S rRNA; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.
| Ranking | All samples | Samples of wounded stems at different times | Samples of different organs | |||
|---|---|---|---|---|---|---|
| Candidate reference genes | Stablity value | Candidate reference genes | Stablity value | Candidate reference genes | Stablity value | |
| 1 | Actin | 0.474 | Actin | 0.223 | α-EF | 0.197 |
| 2 | β-tubulin | 0.474 | β-tubulin | 0.223 | 18S | 0.197 |
| 3 | α-EF | 0.553 | His | 0.389 | β-tubulin | 0.373 |
| 4 | 18S | 0.620 | UBC | 0.469 | Actin | 0.465 |
| 5 | UBC | 0.689 | α-EF | 0.531 | UBC | 0.609 |
| 6 | His | 0.774 | GAPDH | 0.592 | His | 0.831 |
| 7 | GAPDH | 1.063 | 18S | 0.677 | GAPDH | 1.311 |