Table 5.
Primer details of candidate reference genes and target genes. The standard curve is detailed in Supplementary Fig. S2 online.
| Gene symbol | Candidte reference gene | Primer sequence(5′ → 3′) | Amplicon length/bp | E(%) | R2 | Slope | y intercept |
|---|---|---|---|---|---|---|---|
| Actin-Chr5.246 | Actin | F:CCCTGAAAACTGCTCTGCTCTC | 210 | 108.1 | 0.984 | − 3.141 | 22.859 |
| R:CCTCAGGAAATCCCATCTCAAC | |||||||
| α-EF-Chr6.259 | α-Elongation factor | F:CTACAGGAGGCTCTTCCAGGTG | 214 | 111.8 | 0.969 | − 3.069 | 16.523 |
| R:CAGCAATGTGAGAGGTGTGGC | |||||||
| UBC-Chr2.1570 | Ubiquitin conjugating enzyme | F:TTCGCTGGGGGTGTGTTTC | 213 | 100.4 | 0.990 | − 3.312 | 24.037 |
| R:CGGGTTTGGGTCCGTAAGAAG | |||||||
| β-tubulin-Chr4.2695 | β-tubulin | F:TGAGCACCCTCCTGGTCGTA | 195 | 119.7 | 0.990 | − 2.926 | 22.418 |
| R:TGACCTCTGGACACCAACGG | |||||||
| 18S-Chr3.1083 | 18S rRNA | F:GCAGATGGAGGAGGATAGGGTAG | 152 | 108.7 | 0.921 | − 3.129 | 23.255 |
| R:TAAAAGCAGACTTCTCTCCCCC | |||||||
| GAPDH-Chr4.1490 | Glyceraldehyde 3-phosphate dehydrogenase | F:CCAACAAATCATCAAGGACATCAG R:ATGACACAAGCGATTCCATAGAGG | 201 | 125.5 | 0.915 | − 2.831 | 23.202 |
| His-Chr6.1242 | Histone | F:GCCAAGGCAACAGAAAACTCA | 162 | 125.0 | 0.992 | − 2.840 | 41.025 |
| R:ATCCATCACCCTCGTCACCTTA | |||||||
| CHI1-Chr11.863 | Chalcone isomerase 1 | F:CACCACCACAAAGGCTTCACC | 159 | 96.8 | 0.966 | −3.400 | 45.944 |
| R: GCGAGTTCTCTGGCATTCTTTC | |||||||
| PAL1-Chr1.1341 | Phenylalanine ammonia-lyase 1 | F: CTACATCGACGACCCTTGCAG | 242 | 100.6 | 0.984 | − 3.309 | 36.984 |
| R: ATGACCTGCATTCCTTGATCCTG |