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Western blot of purified virions. Gradient-purified PrV(Ka) virions were separated by SDS–13% PAGE under reducing (+ME) or nonreducing (−ME) conditions, transferred to nitrocellulose, and incubated with anti-gN serum. After incubation with peroxidase-conjugated secondary antibody, bound antibodies were detected by chemiluminescence. The positions of molecular mass markers are shown on the left. ME, 2-mercaptoethanol.
