Figure EV3. Depletion of GM-CSF alters the numbers of immune cells infiltrating the joint-footpad.

CHIKV infection (1 × 10⁶ PFU) was performed in the right footpad of wild-type animals. Four days post-infection (dpi), anti-GM-CSF antibodies were given intraperitoneally. Subsequently, numbers of infiltrating CD45⁺ cells, neutrophils, B cells, NKT cells. NK cells, LFA-1⁺CD4⁺ T cells, CD8⁺ T cells, LFA-1⁺CD4⁺ T cells, CD11b⁺Ly6C⁺ cells, CD64⁺MHCII^- cells and CD64⁺MHCII⁺ cells were obtained following immunophenotyping of the joint-footpad at 6 days post-infection (dpi). Data were from biological replicates obtained from two independent experiments. All data are presented as mean ± SD. Data comparisons between the groups were performed with non-parametric Mann–Whitney U test (two-tailed): CD45 + , *P = 0.0139; NKT, *P = 0.0469; NK, *P = 0.0214; LFA-1⁺CD4⁺ T, *P = 0.031; CD8⁺ T, *P = 0.0281; CD64⁺MHCII^-, *P = 0.0186; CD64⁺MHCI⁺, *P = 0.0161.