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. 2024 Feb 8;16(3):641–663. doi: 10.1038/s44321-024-00028-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV1 — (A) Cluster ID annotation with heatmap. Cluster IDs are indicated in rows, while surface marker expression levels are indicated by the columns. The color represents the relative mean signal intensity of a surface marker. Blue and red represent low and high intensity, respectively. (B) Cellular infiltrates in the CHIKV-infected joint-footpad at 6 days post-infection (dpi) were determined with flow cytometry. Brieftly, live CD45⁺ immune cells were gated out before the CD3⁺ (inclusive of CD4⁺ and CD8⁺) T cells, CD3⁺NK1.1⁺ NKT cells, CD11b⁺Ly6G⁺ neutrophils, NK1.1⁺ NK cells, CD11b⁺Ly6C⁺ monocytes and CD64⁺ macrophages were identified. Plots shown are representative of a single CHIKV-infected mouse.