| Antibodies |
|
| Rabbit pAb anti-Caspase-1 p10 |
Santa Cruz |
sc-514; RRID AB_2068895 |
| Goat pAb anti-IL-1 beta/IL-1F2 |
R&D |
AF-401; RRID AB_416684 |
| Mouse mAb anti-Actin |
Abcam |
AB3280; RRID AB_303668 |
| Rat mAb anti-NLRP3 |
R&D |
MAB7578; RRID AB_2889405 |
| Goat pAb anti-COX2 |
R&D |
AF4198; RRID AB_2229909 |
| Rabbit pAb anti-Goat IgG Peroxidase |
Santa Cruz |
sc-2922; RRID AB_656965 |
| Horse pAb anti-mouse IgG Peroxidase |
Cell Signaling |
7076; RRID AB_330924 |
| Goat pAb anti-Rabbit IgG (whole molecule) Peroxidase |
Sigma-Aldrich |
7077; RRID AB_10694715 |
| Rabbit pAb anti-phospho-PKC (pan) |
Cell Signaling |
9371; RRID AB_2168219 |
| Rabbit pAb anti-phospho-PKD |
Cell Signaling |
2051; RRID AB_330841 |
| Rabbit pAb anti-phospho-JNK |
Cell Signaling |
4668; RRID AB_823588 |
|
| Bacterial and virus strains |
|
|
Salmonella enterica Typhimurium SL1344 |
Hoiseth and Stocker46
|
N/A |
|
| Biological samples |
|
| Human blood samples |
From subject cohort – ClinicalTrials.gov ID NCT02719899
|
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
| Acetic Acid |
Millipore Sigma |
5330010050 |
| Arachidonic Acid |
Tocris |
2756 |
| Arachidonic Acid |
Sigma-Aldrich |
10931 |
| ASB-14780 |
Sigma-Aldrich |
SML1913 |
| ATP |
Sigma-Aldrich |
A1852 |
| Celecoxib |
Millipore Sigma |
SML3031 |
| Chloroform |
Millipore Sigma |
288306 |
| CRT0066101 |
Tocris |
4975 |
| Deutered COX and LOX LC-MS Mixture |
Cayman Chemical |
19228 |
| Dulbecco’s Modification of Eagle’s Medium |
Corning |
10–013-CV |
| Dulbecco’s Phosphate-Buffered Saline |
Corning |
21–031-CV |
| Gentamicin |
Millipore Sigma |
G1397 |
| Halt Phosphatase Inhibitor Cocktail (100x) |
Thermo-Fisher |
1862495 |
| Halt Protease Inhibitor Cocktail (100x) |
Thermo-Fisher |
87786 |
| Hexane |
Millipore Sigma |
1043911000 |
| HyClone Fetal Bovine Serum |
Fisher Scientific |
SH3008803 |
| Indomethacin |
Millipore Sigma |
I7378 |
| Isopropanol |
Millipore Sigma |
1027811000 |
| L-Glutamine |
Millipore Sigma |
G7513 |
| LB Broth (Miller) |
Sigma-Aldrich |
L3522 |
| LB Broth with Agar (Miller) |
Sigma-Aldrich |
L3147 |
| Lithocholic Acid |
Millipore Sigma |
L6250 |
| Methanol |
Millipore Sigma |
1.06035 |
| Misoprostol |
Tocris |
2297 |
| N-(p-Amylcinnamoyl)anthranilic acid |
Sigma-Aldrich |
A8486 |
| Nigericin |
Sigma-Aldrich |
N7143 |
| NP-40 (Nonidet P-40 Substitute) |
Boston Bioproducts |
#P-877 |
| Palmitic Acid |
Millipore Sigma |
P0500 |
| Pam3CSK4 |
Invivogen |
tlrl-pms |
| Penicilin Streptomycin Solution, 100x |
Corning |
30–002-CI |
| Pierce™ Lane Marker Reducing Sample Buffer |
Thermo-Fisher |
39000 |
| Prostaglandin D2 |
Cayman Chemical |
12010 |
| Prostaglandin E2 |
Tocris |
2296 |
| Prostaglandin F2alpha |
Tocris |
4214 |
| Prostaglandin I2 |
Tocris |
2989 |
| Sotrastaurim |
Abcam |
ab219867 |
| SP600125 |
Tocris |
1496 |
| TMB Substrate Reagent Set |
BD |
555214 |
| Ultrapure LPS, E. coli O111:B4 |
Invivogen |
tlrl-3pelps |
| U-73122 |
Sigma-Aldrich |
662035 |
|
| Critical commercial assays |
|
| CytoTox 96(R) Non-Radioactive Cytotoxicity Assay |
Promega |
G1780 |
| Clarity Max Western ECL Substrate |
Bio-Rad |
1705062 |
| EnzChek(TM) Direct Phospholipase C Assay Kit |
Invitrogen |
E10215 |
| Human IL-1Beta/IL-1F2 DuoSet ELISA |
R&D Systems |
DY201 |
| Mouse IL-1Beta/IL-1F2 DuoSet ELISA |
R&D Systems |
DY401 |
| Mouse IL-18 Elisa kit |
MBL International |
7625 |
| Pierce BCA Protein Assay Kit |
Thermo Scientific |
23225 |
| PGE2 ELISA kit |
Enzo Life Sciences |
ADI-900–001 |
|
| Deposited data |
|
| Raw data |
This paper |
Mendeley Data: https://doi.org/10.17632/vtpjtgm2tw.1
|
|
| Experimental models: Cell lines |
|
| THP-1 |
ATCC |
ATCC TIB-202 |
|
| Experimental models: Organisms/strains |
| Mouse: C57BL/6 |
Charles Rivers |
N/A |
| Mouse: C57BL/6 NLRP3 Knockout |
Millenium Pharmaceuticals |
N/A |
|
| Software and algorithms |
|
| GraphPad Prism |
Graphpad Software |
www.graphpad.com
|
| ImageJ |
NIH |
imagej.nih.gov/ij/
|
| SIMCA |
Sartorius |
www.sartorius.com
|
