FIG. 2.

In vivo phosphorylation of wild-type and 257 mutant MT. 3T3 cells were transfected with CMV vectors expressing wild-type or 257 mutant MT. Cells were labeled with ³²PO₄ for 2 h. MT immunoprecipitates were resolved on cylindrical gels and then digested with V8 protease in the second dimension. The arrows indicate the positions of the V8 peptides shown in Fig. 1. WT, wild type.