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Viral transformation of F111 rat embryo cellsa
| Polyomavirus | PFU | TFU (foci) | TFU (agar) | PFU/TFU |
|---|---|---|---|---|
| Wild type | 2.7 × 106 | 1.2 × 103 | 1.5 × 103 | ∼2.0 × 103 |
| S257C mutant | 7.8 × 106 | 2.9 × 103 | 4.5 × 103 | ∼2.1 × 103 |
Virus stocks were grown on primary baby mouse kidney cells. Plaque titers (PFU) were determined on NIH 3T3 cells and transforming titers (TFU) were determined on F111 cells (24).
