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. 2024 Jan 13;13(3):278–292. doi: 10.1093/stcltm/szad091

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© The Author(s) 2024. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Image analysis pipeline. (a) Representative raw images of large and small microwells with high and low microtissue movement. (b) For each image, the microwell grid was segmented and (c) a microtissue probability map of all pixels was generated. (d) Individual spheroid objects were identified and (e) overlayed on the microwell grid. (f) Microtissue size distribution in a well. (g-l) 24 well plate digitalization for A400 and A800 and individual well tracking over time. Each microwell is colored according to whether it contains a microtissue or not.