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. 2024 Jan 13;13(3):278–292. doi: 10.1093/stcltm/szad091

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© The Author(s) 2024. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Biological characterization of microtissues and ectopic explants. (a) Protocol overview and timeline. (b-g) mRNA quantification of differentiation markers. (i) DNA quantification on day 21. Histological staining of microtissues on day 21 showing (j) hematoxylin-eosin, (k) Alcian blue, and (l) safranin O. Scale bars = 200 μm. (m) Mineralisation after 4 weeks ectopic implantation of microtissue constructs measured by microCT. Histological staining of explants showing (n) hematoxylin-eosin, and (o) safraninO-fastgreen staining of representative explants. Scale bars = 1 mm. (p) Glycosaminoglycan (GAG) quantification. (q) Sulphated glycosaminoglycan (sGAG) quantification. (r) Explant volumes. (s) Explant percentage mineralisation.