Table 1.

Summary statistics on microtissue size distribution and percentages of empty microwells.

Platform	Day	Microtissues identified	Microwells per well	Percentage empty microwells	Percentage filled microwells	Volume per microtissue (mm³)	D10 (mm³)	D50 (mm³)	D90 (mm³)	Span	Predicted tissue volume (mm³)
A400	7	14867	1200	8.82	91.18	0.0015	0.0009	0.0017	0.0033	1.4571	1.6014
	14	13210	1200	16.38	83.62	0.0017	0.0010	0.0020	0.0054	2.2674	1.7358
	21	12647	1200	21.11	78.89	0.0021	0.0012	0.0024	0.0070	2.4076	1.9432
A800	7	4014	300	10.62	89.38	0.0059	0.0033	0.0068	0.0160	1.8612	1.5851
	14	3574	300	26.80	73.20	0.0066	0.0036	0.0084	0.0162	1.5071	1.4487
	21	3158	300	29.23	70.77	0.0093	0.0048	0.0118	0.0318	2.2735	1.9731

Particle distribution parameters D10, D50, and D90 are derived from the ordered cumulative size distribution and indicate the microtissue size cutoff point where 10%, 50%, and 90% of microtissues respectively are below this size. The span, calculated as $\mathrm{s}\mathrm{p}\mathrm{a}\mathrm{n}=(\mathrm{D}90-\mathrm{D}10)/\mathrm{D}50$ is used to describe the distribution range. The predicted tissue volume per well is calculated as the amount of theoretical microwells per well times the average volume per microtissue, corrected for the amount of empty microwells.